Consequently, this subpopulation of cells is named CSC or tumor stem cells (TSC; refs. However, there are no reports about the existence and function of SP cells in nasopharyngeal carcinoma (NPC) cells thus far.
Side population (SP) cells have been isolated from several solid tumors. Hoechst 33342 is a cell-permeable DNA-specific bisbenzimidazole dye. Hoechst side population (SP) analysis has proven to be a valuable technique for identifying and sorting stem and early progenitor cells in a variety of tissues and species. AU - Shimoda, Masayuki. After some of the sorted cells were cultured for several days (<1 week), we harvested 1 × 10The differentiation assay was done 18 days after cell sorting. Side population (SP) cells have been isolated from several solid tumors. Furthermore, the stem cell‐specific presence of aldehyde dehydrogenase isoform 1 can be used for CSC labeling. I have read and accept the Wiley Online Library Terms and Conditions of UsePro-inflammatory modification of cancer cells microsurroundings increases the survival rates for rats with low differentiated malignant glioma of brain, Generation of multidrug resistant human tissues by overexpression of the ABCG2 multidrug transporter in embryonic stem cells, Cancer Stem Cells and Multi-drug Resistance by Flow Cytometry, Application of fluorescent dye substrates for functional characterization of ABC multidrug transporters at a single cell level, Cell sorting in cancer research—Diminishing degree of cell heterogeneity, Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Isolation and Applications of Prostate Side Population Cells Based on Dye Cycle Violet Efflux, All these characteristics are similar to normal adult stem and even embryonic stem cells. AU - Okada, Yasunori. HSCs represent a paradigm for the study of stem-cell biology, because robust methods for prospective isolation of HSCs have facilitated rigorous characterization of these cells. Please check your email for instructions on resetting your password.
This study tested the hypothesis that side population hematopoietic stem cells (SP-HSC) would decrease with aging, correlating with IGF-1 and IL-6 levels and increases in bone marrow fat. Mitoxantrone was added the following day in a concentration gradient and repeated in four wells. For decades, tumor development has been regarded as a multistep process, in which progressive genetic alterations drive the transformation of normal human cells into highly malignant derivatives. Hoechst side population (SP) analysis has proven to be a valuable technique for identifying and sorting stem and early progenitor cells in a variety of tissues and species. Results of radiation and chemotherapy drug sensitivity assays of SP and NSP cells and Western blot of SMO.
In some cases, CSC may arise from mutational transformation, in which normal stem cells gain a cancer phenotype, whereas in other cases, mutations that enhance self-renewal may cause committed progenitor cells or differentiated cells to adopt properties of CSCs (
According to the CSC theory, not all cancer cells are created equal ( We observed a strong tumorigenesis ability of SP cells following The concept of cancer stem cell (CSC) was introduced many years ago to explain tumor cell heterogeneity, and recent studies suggest that CSC may be responsible for tumorigenesis and contribute to some individuals' resistance to cancer therapy. Y1 - 2018. Hematological deficiencies increase with aging leading to anemias, reduced hematopoietic stress responses and myelodysplasias. The data showed that SP cells still contain 9.6% Hoechst 33342 dull cells, whereas NSP cells only contain 0.4% ( We also found that both SP and NSP cells had morphologic changes when they were maintained in UltraMEM (containing 5% FBS, 10 ng/mL bFGF, and EGF; It has been reported that side‐population (SP) cells with the ability to efflux the DNA‐binding dye Hoechst 33342 have stem cell–like properties and that the SP phenotype accurately represents a quiescent and immature stem cell population in the adult bone marrow. DyeCycle Violet can be substituted for Hoechst 33342 when performing side population analysis on a cytometer with a violet laser source. Self-renewal and differentiation are properties of stem cells that allow them to generate additional CSC and phenotypically diverse cancer cells with a limited proliferative potential. DyeCycle Violet is a cell‐permeable DNA binding dye with a chemical structure similar to Hoechst 33342, but with a longer excitation maximum. Recently, the detection of side population (SP) cells, which have the ability to strongly efflux Hoechst 33342 (Ho) fluorescence dye, has attracted attention as a method of stem cell isolation. The culture medium was then replaced with different concentrations of mitoxantrone.
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We know that several pathways that regulate normal stem cell self-renewal cause neoplastic proliferation when deregulated by mutations, such as WNT/β-catenin, SHH, Notch, and PTEN (